Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8) ; CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.
Target |
Bid (pS65) |
Reactivity |
Human |
Host |
Rabbit |
Clonality |
Polyclonal |
Tested Applications |
WB, IHC |
Recommended dilutions |
Optimal dilutions/concentrations should be determined by the end user. |
Immunogen |
KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid. |
Purification |
Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Isotype |
IgG |
Conjugation |
Unconjugated |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
Swiss Prot |
P55957
|
Buffer |
PBS containing 0.09% sodium azide. |
UNSPSC Code |
12352203 |
Availability |
Shipped within 5-10 working days. |
Note |
This product is for research use only. |