Human CD45RA is expressed on all cells of hematopoietic origin, except erythrocytes. CD45RA is a transmembrane tyrosine phosphate which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RA isoform predominates on naive/resting T cells and medullary thymocytes.
| Target |
CD45RA |
| Reactivity |
Human |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
FCM |
| Recommended dilutions |
FCM: 20 µl/1 million cells. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Tissue / cell preparation (Human). Fresh human lymph node lymphocytes depleted of surface immunoglobulin positive cells were used as the immunogen. This antigen is a single chain glycoprotein which is expressed on B cells, monocytes, and T cell subsets. |
| Purification |
Affinity Chromatography |
| Form |
Liquid |
| Isotype |
IgG1 |
| Conjugation |
FITC |
| Specificity |
The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral Tlymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100mAb as a phenotypic marker to discriminate T cell subsets. |
| Storage |
Aliquot and store in the dark at 2°C to 8°C. Avoid exposure to light. |
| Molecular Weight |
220 kDa |
| Swiss Prot |
P08575
|
| GeneID |
5788
|
| Enzyme Commission Number |
EC 3.1.3.4 |
| Buffer |
The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 3-5 working days. |
| Note |
This product is for research use only. |