Mouse monoclonal antibody to CD3 (APC).
| Target |
CD3 |
| Reactivity |
Human |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
IF/ICC, FCM |
| Recommended dilutions |
Optimal dilutions/concentrations should be determined by the end user. |
| Purification |
Purified by affinity chromatography. |
| Form |
Liquid |
| Isotype |
IgG2a |
| Conjugation |
APC |
| Specificity |
Recognises human CD3. |
| Storage |
Store at 4 °C. Do not freeze. |
| Swiss Prot |
P04234
|
| GeneID |
915
916
917
919
|
| Gene Symbol |
CD3D, CD3E, CD3G, CD247 |
| Buffer |
PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide. |
| UNSPSC Code |
12352203 |
| Directions for use |
- Take 100 µl peripheral blood anticoagulated by EDTA and add to the bottom of a 5 ml tube.
- Add 10 µl labeled antibody to the bottom of the flow tube and mix with the whole blood. Allow to stand for 20 minutes at room temperature in the dark.
- Add 2 ml RBC lysis buffer, mix, then incubate for 10 minutes in the dark to dissolve red blood cells.
- Centrifuge the sample tube at 1000 RPM for 5 minutes. Discard the supernatant.
- Add 2 ml PBS to resuspend and wash the cells, then centrifuge at 1000 RPM centrifugation for 5 minutes. Discard the supernatant.
- Add 0.5 ml PBS to resuspend the cells, and detect by flow cytometry (sample should be measured on the same day, or can be fixed overnight at 4 °C then measured).
|
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |