TransIT®-VirusGEN™ Transfection Reagent is designed to enhance delivery of packaging and transfer vectors to adherent and suspension HEK 293 cell types to increase recombinant adeno-associated virus (AAV) and lentivirus production.

Adeno-associated virus (AAV) is a nonenveloped, single stranded DNA virus from the Paroviridae family notable for its lack of pathogenicity, low immunogenicity and ability to infect both dividing and quiescent cells. Because AAV is replication-defective in the absence of adeno or helper proteins and is not implicated in any known human diseases, it is widely considered a safe gene delivery vehicle for in vivo and in vitro applications. Accordingly, recombinant AAV has become an invaluable tool for gene therapy and the creation of isogenic human disease models.
Lentivirus is an enveloped, single-stranded RNA virus from the Retroviridae family capable of infecting both dividing and non-dividing cells. Combined with an efficient host-genome integration mechanism and the ability to pseudotype the virus, this capability makes recombinant lentivirus a central gene delivery tool for robust and stable transgene expression in target cells.
The TransIT®-VirusGEN™ Transfection Reagent enables the generation of both high titer AAV and lentivirus in adherent or suspension HEK 293 cell types. With salient features including: high efficiency DNA delivery, culture format versatility and streamlined virus generation workflows, TransIT®-VirusGEN™ is an ideal reagent for scientists utilizing a variety of virus platforms to accelerate their research.