This unique combination of proprietary buffer, stabilizers, and AccuFast™ Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, and specificity, along with a robust fluorescent signal using fast, or conventional, cycling protocols with SYBR® Green qPCR

AccuFast™ Taq DNA polymerase is a key component of this reaction mix. This hot-start Taq contains a unique mixture of monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step (>48 hours at room temperature). Similar to our AccuStart™ Taq DNA polymerase, these antibodies are irreversibly inactivated during the initial PCR denaturation step. However, unlike other antibody hot-start polymerases, activation of AccuFast™ Taq is instantaneous at 95°C. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Replication of fragments up to 200bp is complete in less than 20 seconds at 60°C.

Kits are available for all real-time PCR instrument platforms including those requiring normalization with ROX™ reference dye. Platforms not requiring passive reference dye include Roche LightCycler® 480, Bio-Rad® iQ™, MyiQ™, iQ™5, Opticon®, and Chromo4™, Corbett Rotor-Gene®, Eppendorf® Mastercycler®, and Cepheid® SmartCycler®. Kits with Low ROX™ reference dye are for use with platforms including AB 7500 and Stratagene® Mx™. Kits with ROX™ reference dye are for use with platforms including AB 7000, 7300, 7700, 7900, and StepOne™.

Highly specific amplification is crucial to successful qPCR with SYBR® Green I dye technology because this dye binds to and detects any dsDNA generated during amplification.