The qScript microRNA cDNA Synthesis Kit has been designed to convert miRNAs into cDNA starting from total RNA or RNA preparations pre-enriched for miRNAs

The kit comes complete with a Human Positive Control Primer that can be used to quantitate the small nucleolar RNA SNORD44 which is ubiquitously expressed in most human tissues. In addition, the kit contains 20% extra Poly(A) Tailing Buffer and MicroRNA cDNA Reaction Mix to accommodate the use of no poly(A) polymerase and no reverse transciptase control reactions. Individual microRNAs are quantitated in real-time qRT-PCR amplification reactions using the PerfeCTa microRNA Assays along with the PerfeCTa Universal PCR Primer (specific to the unique sequence of the oligo-dT adapter primer) and the PerfeCTa SYBR Green SuperMix family of products (see the relevant products section below). The pre-designed and validated PerfeCTa MicroRNA Assays provide maximum sensitivity and specificity in qRT-PCR amplifications. For a complete list of available microRNA assays please visit www.Quantabio.com/microRNA.

Contents:
- Poly(A) Tailing Buffer (5X)
- Poly(A) Polymerase
- MicroRNA cDNA Reaction Mix
- qScript Reverse Transcriptase
- PerfeCTa Universal PCR Primer
- PerfeCTa Human Positive Control Primer
- Nuclease-Free Water

Storage conditions:
The kit components are stable for one year when stored at -20°C.

Precautions and Disclaimer:
For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

miRNAs are not polyadenylated in nature. With the qScript microRNA cDNA Synthesis Kit miRNAs are polyadenylated in a poly(A) polymerase reaction. qScript Reverse Transcriptase and other necessary reagents for cDNA synthesis are subsequently added to convert the poly(A) tailed microRNAs into cDNA using an oligo-dT adapter primer. The adapter primer has a unique sequence at its 5’ end which allows amplification of cDNAs in real-time qRT-PCR reactions.