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产品名称 illustra Single Cell GenomiPhi DNA Amplification Kit, GE Healthcare
产品货号 29-1080-39
产品价格 现货询价，电话：010-67529703
产品规格
产品品牌 vwr
产品概述
产品详情
Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.

  • Whole Genome Amplification of genomic DNA from as little as a single cell
  • Novel clean-up process supresses background allowing sensitivity down to 1 fg of input DNA
  • Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
  • Amplifications can be completed in less than three hours with less than 15 minutes hands-on time
  • Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification

Genomic analysis from single cells is an emerging area as researchers focus in on differences within cell populations

The amount of genomic DNA needed to carry out a full range of testing has impacted progress, and while techniques become more sensitive, sharing a single cell's DNA across platforms is not possible. Single Cell GenomiPhi DNA Amplification kit has been optimized to wholly amplify genomic DNA from as little as a single cell, generating micrograms of high quality DNA for use in downstream applications.

At very low levels of input DNA, successful amplification relies on reagent quality. Single Cell GenomiPhi has been developed with this in mind. Lysis reagents are optimized to fully release genomic DNA from the cell and subsequently denature the DNA to enable optimal amplification and coverage. Background amplification, often associated with Multiple Displacement Amplification (MDA), is suppressed throughout the incubation so that only input DNA is amplified.

High quality reagents and an optional, proprietary enzymatic clean-up step in the protocol ensure that any potential DNA contaminants introduced during set-up are removed before each individual reaction. In addition, our manufacturing processes, including UV and enzymatic reagent cleanup, help to ensure that all kits are free from any detectable DNA contamination and enable sensitivity of amplification down to 1 fg of gDNA.

Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity. DNA from single cells can be reproducibly and reliably amplified for use in many downstream applications. Single Cell GenomiPhi has been successfully used to generate template for SNP array genotyping, array CGH, and next-generation sequencing.

Ordering information: Kit contains Single Cell GenomiPhi Reaction buffer 1 x 1.1mL, Single Cell GenomiPhi Enzyme mix 1 x 100 µL, Single Cell GenomiPhi Amplification mix 1 x 100 µL, Single Cell GenomiPhi DTT Solution1 x 100 µl, Single Cell GenomiPhi Lysis buffer 1 x 200 µl and Single Cell GenomiPhi Neutralisation buffer 1 x 200 µl.

Packaging: Store the kit at -70º C. The enzyme mix must be stored at -70º C; all other components may be stored at -20° C.
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