世联博研公司现货供应vwr品牌订货号为25-6400-10的illustra TempliPhi 100/500 Amplification Kits, GE Healthcare产品

产品名称	illustra TempliPhi 100/500 Amplification Kits, GE Healthcare
产品货号	25-6400-10
产品价格	现货询价，电话：010-67529703
产品规格
产品品牌	vwr
产品概述
产品详情	(High-throughput) preparation of circular DNA templates for cycle sequencing, cloning, and transformation in 4-8 h depending on starting material, 16-18 h for the high-throughput material. Use amplified DNA for cycle sequencing without purification Low-to medium-throughput: Small DNA vector preparation Generate microgram quantities of template DNA from picogram amounts of starting material Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA, 1–10 ng Simple protocol reduces time, labor, and consumables needed for template preparation, and workflow enables easy automation TempliPhi Kits use a unique process to prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30°C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies. Two different types of TempliPhi DNA Amplification Kits are available to meet different throughput requirements. illustra TempliPhi DNA Amplification Kit for 2000 reactions contains premixed components and generates templates after overnight incubation. The 100- and 500-reaction kits contain separate, unmixed components for enhanced stability. The illustra TempliPhi DNA Sequencing Template Amplification Kit has a distinct protocol and workflow compared to the 100/500 reaction size. Procedures for each kit are not interchangeable. Prior optimization is needed. The TempliPhi premix is stable at -70°C for 6 months from the date of manufacture. The denature buffer and positive control DNA are stable at -70°C or -15°C to -30°C for 12 months from the date of manufacture. Ordering information: Sample Buffer (white cap) 0.5 mL; Reaction Buffer (Blue cap) 0.5 mL; Enzyme mix (yellow cap) 20 µL; Positive control DNA (pUC19, 2 ng/µl) 50 µL. TempliPhi premix 20 mL; Denature buffer 10 mM Tris-HCl, pH 8.2, 0.5 mM EDTA, 20 mL; Positive control pUC19 DNA 10 ?L, 2 ?g/mL.
产品资料