Accurate quantification of the number of amplifiable library molecules is the most critical step in the NGS workflow in obtaining high quality read data with next-generation sequencing technologies. The PerfeCta® NGS Library Quantification Kit uses real-time PCR to specifically quantify library molecules that possess the appropriate adapter tag at each end. These are the suitable template molecules for either emulsion PCR used for the Ion Torrent platform or Bridge PCR used for Illumina NGS platforms.

PerfeCta NGS Quantification kits simplify the library quantification process by providing stabilized, pre-diluted standards, pre-qualified primer sets, and an optimized dilution buffer for your NGS library samples. This minimizes pipetting errors and ensures reproducible and precise qPCR results, even with dilute samples. The robust qPCR performance of PerfeCta® SYBR Green SuperMix provides accurate quantification of NGS libraries with varying fragment sizes or GC content. Kits are available to support all major qPCR instrument platforms.

Kit components are stable for 2 years when stored in a constant temperature freezer at -20°C and protected from light. For convenience, they may be stored unfrozen at +2 to +8ºC for up to 6 months. Repeated freezing and thawing will not affect product performance.