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| Product Name | Active Recombinant Human Interleukin-6 |
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| Description | Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal Free product. Recombinant human Interleukin-6 is an important pro-inflammatory and anti-inflammatory cytokine expressed by many types cell including: T and B cells, macrophages, endothelial cells, fibroblasts, monocytes, keratinocytes and certain tumour cells. It is a multifunctional cytokine that modulates several physiologic processes such as haematopoiesis, stimulation of immunoglobulin synthesis, maturation and activation of B cells, differentiation of T lymphocytes and regulation of the hepatic acute-phase response. IL-6 is also produced in muscle, is discharged into the bloodstream after muscle contraction and acts increasing the breakdown of fats and improving insulin resistance. IL-6 induces signalling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signalling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R. Trans-signalling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Recombinant human Interleukin-6 is a polypeptide chain containing 183 amino acids (30 - 212 of P05231 IL6_HUMAN) and 10 aa Histidine-based tag. It as a predicted molecular mass of 22.2 kDa, however as result of potential glycosylation, the recombinant protein could migrate with an apparent molecular mass of 23-24 kDa in SDS-PAGE gel. Purity >97% by SDS-PAGE gel |
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| Size | 1 µg, 5 µg, 10 µg, 20 µg, 50 µg |
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| Concentration | n/a |
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| Applications | Functional studies, Cell culture, Western Blot. |
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| Other Names | Interleukin 6 (interferon, beta 2) |
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| Gene, Accession, CAS # | UniProt: P05231 |
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| Catalog # | RF0032 |
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| Order / More Info | Active Recombinant Human Interleukin-6 from AGRENVEC |
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| Product Specific References | - Fonseca, J.E. et al. (2009) Interleikin-6 as a key player in systemic inflammation and joint destruction. Autoimmun. Rev., 8:538-542. - Kamimura, D. et al. (2003) Il-6 signal transduction and its physiological roles: signal orchestration model. Rev. Physiol. Biochem. Pharmacol., 149: 1-38. - Kanazawa, T. et al. (2007) Interleukin-6 directly influences proliferation and invasion potential of head and neck cancer cells. Eur. Arch. Otorhinolaryngol., 264:815-821. - Kishimoto, T. (1989) Te biology of interleukin-6. Blood, 74:1-10. - Febbraio, M. A. and B. K. Pedersen (2002) Muscule-derived interleukin-6: mechanisms for activation and possible biological roles. FASEB J., 16: 1335. - Van Sninck, J (1990) Interleukin-6: an overview. Annu. Rev. Immunol., 8:253-78. - Hong, D. et al. (2007) Interleukin-6 and its receptor in cancer: implications for translational therapeutics. Cancer. Nov 1; 110(9):1911-28. |
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