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Product Name | Proteinase K Solution 20mg/ml |
Description | Proteinase K is classified as a non-specific serine protease. It is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Applications: • Isolation of high molecular weight DNA • Isolation of plasmid and genomic DNA • Isolation of RNA • Inactivation of RNase and DNase activities Advantages: • Ready to use, provided in liquid form containing 40% glycerol. • Smaller contamination risk with liquid form. • Can be stored at -20°C still having the enzyme in liquid form. • Excellent stability Notes: •Proteinase K is not inactivated by metal-chelating agents like EDTA, by thiol-reactive reagents (such as monoiodoacetic acid, p-chloromercuribenzoate) or by specific trypsin and chymotrypsin inhibitors. Phenylmethylsulfonyl fluoride and diisopropyl phosphorofluoridate completely inhibit the enzyme. •Proteinase K is stable over a wide pH range: 4-12.5. •Raising the temperature of the reaction from 37°C to 50°-65°C can increase t |
Size | 1ml, 5ml, 10ml |
Concentration | n/a |
Applications | n/a |
Other Names | EC=3.4.21.64; PROK; Endopeptidase K; Tritirachium Alkaline Proteinase |
Gene, Accession, CAS # | n/a |
Catalog # | P9101 |
Price | |
Order / More Info | Proteinase K Solution 20mg/ml from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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