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| Product Name | DNA Polymerase I, Large (Klenow) Fragment |
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| Description | DNA Polymerase I, Large (Klenow) Fragment (about 68 kD) is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'→5' exonuclease activity, but has lost 5'→3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini. It is applicable to DNA sequencing by the Sanger dideoxy method, fill-in of 5? overhangs to form blunt ends, removal of 3? overhangs to form blunt ends, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols. Theoretical protein molecular weight is 68000 daltons. Error Rate: ~ 18x10-6 bases |
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| Size | 200 U / 1000 U / 5000 U |
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| Concentration | n/a |
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| Applications | Blunting, DNA Sequencing , Polymerases for DNA Manipulation, RT-qPCR, RT-PCR and cDNA Synthesis, RT-PCR & cDNA Synthesis, PCR |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | AGE-0004 |
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| Order / More Info | DNA Polymerase I, Large (Klenow) Fragment from ACCEGEN BIOTECH |
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| Product Specific References | n/a |
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