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Product Name | nth, 1-211aa, E.coli, His tag, E.coli |
Description | Purity: > 90% by SDS - PAGE Format: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF. Endonuclease III, also known as nth, is a DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. Recombinant E.coli nth protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
Size | 250ug, 50ug, 10ug |
Concentration | n/a |
Applications | SDS-PAGE |
Other Names | Endonuclease III, b1633, JW1625, DNA-(apurinic or apyrimidinic site) lyase, Endonuclease-III |
Gene, Accession, CAS # | NP 416150 |
Catalog # | ATGP1052 |
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Order / More Info | nth, 1-211aa, E.coli, His tag, E.coli from ATGen |
Product Specific References | n/a |
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