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Product Name | Nitric Oxide Fluorometric BioAssay™ Kit |
Description | Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. Since NO is rapidly converted to nitrite (NO2−) and nitrate (NO3−), the total concentration of nitrite and nitrate is used as a quantitative measure of NO production. BioAssay™ Nitric Oxide Fluorometric Assay Kit provides an accurate and convenient measurement of total nitrate/nitrite concentration in a simple two-step process. In the first step nitrate is converted to nitrite by nitrate reductase. In the second step, nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The fluorescent intensity is proportional to the total nitric oxide production. The kit has been tested with culture media, plasma, and tissue homogenates. Kit Components: N2577-05A: Assay Buffer 1x30ml N2577-05B: Enzyme Co-factors 1x1 vial N2577-05C: Nitrate Reductase 1x1 vial N2577-05D: Nitrate Standard 1x1 vial N2577-05E: DAN Rea |
Size | 2x96 tests |
Concentration | n/a |
Applications | n/a |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | N2577-05 |
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Order / More Info | Nitric Oxide Fluorometric BioAssay™ Kit from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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