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| Product Name | (Leu24)(Ala31)IGF-I, recombinant protein (Receptor Grade) |
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| Description | Product Specifications [Leu24][Ala31]IGF-I: [Leu24][Ala31]IGF-I is an analog of human IGF-I with a substitution of Leu for Tyr at position 24 and an Ala for Tyr at position 31 of the human IGF-I sequence. [Leu24][Ala31]IGF-I has greatly reduced binding to the Type 1 IGF receptor compared to IGF-I, and a lower affinity than [Leu24]IGF-I or [Leu60]IGF-I. However [Leu24][Ala31]IGF-I has normal binding to acid-stripped human serum binding proteins. Consequently [Leu24][Ala31]IGF-I is an extremely useful analog of IGF-I for investigating IGF actions largely independent of binding to the Type 1 IGF receptor. Source: [Leu24][Ala31]IGF-I is produced recombinantly as a fusion protein in E.coli using a patented expression system. The fusion protein containing [Leu24][Ala31]IGF-I is refolded, and after the leader peptide is removed, the [Leu24][Ala31]IGF-I product is purified to Receptor Grade using several chromatography steps.Purity: > 95% (as determined by HPLC and N-terminal sequence analysis)Molecular weight:Theoretical : 7507 daltons. Confirmed by Mass Spect rometry.Appearance:Lyophilized white powderN-terminal sequence analysis:5 residues > 95% single sequence Endotoxin (LAL): 750 ng/ml. IGF binding protein assay - ED50 2000 ng/ml.State: Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25kPa).Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g. < 1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.Storage/Stability: At least 2 years at 2-4°C (lyophilized) Peptide can be biotinylated by ibt upon request. This is a Gropep specification. ibt is authorized distributor of GroPep. |
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| Size | 20 µg |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | [Leu24][Ala31] Insulin-like Growth Factor-1; [Leu24][Ala31]IGF-1 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ADU020 ADU100 |
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| Order / More Info | (Leu24)(Ala31)IGF-I, recombinant protein (Receptor Grade) from IMMUNOLOGICAL & BIOCHEMICAL TESTSYSTEMS GmbH |
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| Product Specific References | n/a |
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