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| Product Name | Human BD-1 / beta Defensin-1 ELISA kit |
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| Description | This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for BD-1 / beta Defensin-1 has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any BD1 / beta Defensin-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for BD-1 / beta Defensin-1 is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of BD-1 / beta Defensin-1 bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm.The concentration of BD-1 / beta Defensin-1 in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: serum, plasma, cell culture supernatants. Range: 1.56 - 100 pg/ml. Sensitivity: 0.8 pg/ml. Time: 4 hours • Defensins are 2-6 kDa, cationic, microbicidal peptides active against many Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses, containing three pairs of intramolecular disulphide bonds. On the basis of their size and pattern of disulphide bonding, mammalian defensins are classified into alpha, beta and theta categories. Every mammalian species explored thus far has beta-defensins. In cows, as many as 13 beta-defensins exist in neutrophils. However, in other species, betadefensins are more often produced by epithelial cells lining various organs (e.g. the epidermis, bronchial tree and genitourinary tract). Defensins are produced constitutively and/or in response to microbial products or proinflammatory cytokines. Some defensins are also called corticostatins (CS) because they inhibit corticotropin-stimulated corticosteroid production. The mechanism(s) by which microorganisms are killed and/or inactivated by defensins is not understood completely. However, it is generally believed that killing is a consequence of disruption of the microbial membrane. The polar topology of defensins, with spatially separated charged and hydrophobic regions, allows them to insert themselves into the phospholipid membranes so that their hydrophobic regions are buried within the lipid membrane interior and their charged (mostly cationic) regions interact with anionic phospholipid head groups and water. Subsequently, some defensins can aggregate to form `channel-like' pores; others might bind to and cover the microbial membrane in a `carpet-like' manner. The net outcome is the disruption of membrane integrity and function, which ultimately leads to the lysis of microorganisms. Some defensins are synthesised as propeptides which may be relevant to this process. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | beta Defensin-1, BD-1 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ARG80905 |
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| Order / More Info | Human BD-1 / beta Defensin-1 ELISA kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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