| 产品详情 |
| Edit |   |
|---|
| Product Name | High Sensitive Human Fetuin-A ELISA Assay Kit |
|---|
| Description | This Eagle Biosciences High Sensitive Human Fetuin-A ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human Fetuin-A in serum samples. The assay utilizes the two-site ``sandwich'' technique with two selected goat anti-human Fetuin-A polyclonal antibodies that bind to different epitopes of human Fetuin-A. Assay standards, controls and prediluted patient serum samples containing human Fetuin-A are added to microtiter wells of a microplate that was coated with a high affinity polyclonal goat anti-human Fetuin-A antibody. After the first incubation period, the antibody on the wall of microtiter well captures human Fetuin-A in the sample and unbound proteins in each microtiter well are washed away. Then a horseradish peroxidase (HRP)-conjugated polyclonal anti-human Fetuin-A antibody is added to each microtiter well and a ``sandwich'' of ``capture antibody - human Fetuin-A - HRP-conjugated tracer antibody'' is formed. The unbound tracer antibody is removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to the Fetuin-A on the wall of the microtiter well is directly proportional to the amount of Fetuin-A in the sample. A standard curve is generated by plotting the absorbance versus the respective human Fetuin-A concentration for each standard on point-to-point or cubical scales. The concentration of human Fetuin-A in test samples is determined directly from this standard curve. Sample Types: serum. Range: 0 -4343 ng/mL. Sensitivity: 40 ng/mL. Time: 3 hours • Fetuin-A, also known as alpha-2-HS glycoprotein, is a 59 kDa glycoprotein that consists of two amino-terminal cystatin domains and a smaller carboxyl-terminal domain. Fetuin-A is synthesized by the liver and secreted into blood stream, where its concentration in adult mammals ranges from 0.5 - 1.5 g/L. Fetuin-A occurs in high serum concentration during fetal life and involves in protease inhibitory activities and development-associated regulation of calcium metabolism and osteogenesis. It accumulates in bones and teeth as a major fraction of noncollagenous bone proteins. Biologically, studies have demonstrated that Fetuin-A is the major calcification inhibitor found in serum, where it interferes with calcium salt precipitation. A recent study has indicated that Fetuin-A level drops in uremic patients on hemodialysis in comparison to normal healthy controls. The low Fetuin-A level may be associated with a higher cardiovascular mortality in patients on dialysis. |
|---|
| Size | 1 x 96 well |
|---|
| Concentration | n/a |
|---|
| Applications | RUO |
|---|
| Other Names | Human Fetuin-A, sensitive |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | HFA31-K01 |
|---|
| Price | |
|---|
| Order / More Info | High Sensitive Human Fetuin-A ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|