产品名称 EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence from BIOVISION, INC.
产品货号 K416-100
产品价格 现货询价,电话:010-67529703
产品规格 100 assays
产品品牌 BIOVISION, INC.
产品概述
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Product NameEZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence
DescriptionPalmitoylation occurs when fatty acids, such as Palmitic Acid are covalently attached to the side chains of cysteine (and less frequently to serine, threonine side chains) in proteins. This type of post-translational modification greatly affects cellular trafficking, compartmentalization, and membrane tethering. Palmityltransferase (PAT), the enzyme responsible for this modification, transfers a palmitate moiety from palmitoyl-CoA to the thiol group of cysteine in the target protein. Compared to myristoylation, palmitoylation is reversible and the reverse reaction is catalyzed by thioesterases. The Palmitoylation/Depalmitoylation cycle plays an important role when modified protein shuttles between cellular compartments. PAT mutations are associated with many neurological diseases and cancer progression. BioVision offers EZClick™ Palmitoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of palmitoylated proteins. The kit uses a modified Palmitic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation. This post translational modification can be followed by click reaction with an azide-containing dye. The assay kit offers a powerful method for imaging localization, trafficking, and dynamics of Palmitoylated proteins, or detection by FACS for quantitative studies. Labeled Palmitoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.
Size100 assays
Concentrationn/a
Applicationsfor labeling and detection of Palmitoylated proteins
Other NamesEZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Red, EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence, EZClick™ Palmitoylated Protein Kit (FACS/Microscopy), Red Fluorescence, Palmitoylated Protein Assay Kit, EZClick™ Palmitoylated Protein Kit (FACS/Microscopy), Red, EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), EZClick™ Palmitoylated Protein Assay (FACS/Microscopy), Red Fluorescence, EZClick™ Palmitoylated Protein Assay Kit, Red, Palmitoylated Protein Assay
Gene, Accession, CAS #n/a
Catalog #K416-100
Price
Order / More InfoEZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence from BIOVISION, INC.
Product Specific Referencesn/a
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