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Product Name | Anti-Thyroid Peroxidase Antibody BioAssay™ ELISA Kit (Human) |
Description | Sample Type: Serum, plasma and other biological fluids. Intended Use: Sandwich ELISA, Double Antigen Sensitivity: <1.875ng/ml Range: 3.125-200ng/ml Specificity: Human Test Principle: This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Thyroid peroxidase was pre-coated onto 96-well plates. And the Biotin-labeled Thyroid peroxidase was used as detection antigen. The standards, test samples and Biotin-labeled Thyroid peroxidase were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the anti-Thyroid peroxidase antibody amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentrati |
Size | 48 tests, 96 tests |
Concentration | n/a |
Applications | ELISA |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | 369744 |
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Order / More Info | Anti-Thyroid Peroxidase Antibody BioAssay™ ELISA Kit (Human) from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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