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| Product Name | Caspase 3/7 Activity Apoptosis Assay Kit (green fluorescence) |
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| Description | Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Green Fluorescence*. Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring Caspase 3 activation. Caspase 3 is widely accepted as a reliable indicator for cell apoptosis since the activation of caspase-3 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses Z-DEVD-Rh 110-DVED-Z as a fluorogenic indicator for caspase-3 activity. Cleavage of Rh 110 peptides by caspase 3 generates strongly fluorescent Rh 110 that is monitored fluorimetrically at 520-530 nm with excitation of 480-500 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays. Using 100 ul of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 200 assays. Using 25 ul of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 800 assays. |
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| Size | 200 tests |
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| Concentration | n/a |
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| Applications | Biossay, Fluorometric |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 22796 |
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| Order / More Info | Caspase 3/7 Activity Apoptosis Assay Kit (green fluorescence) from AAT BIOQUEST, INC. |
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| Product Specific References | n/a |
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