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| Product Name | IGFBP-1 ELISA |
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| Description | The Eagle Biosciences ELISA for IGFBP-1 is a so-called Sandwich-Assay. It utilizes two specific and high affinity antibodies for this protein. The IGFBP-1 in the sample binds to the immobilized first antibody on the microtiter plate. In the following step, the biotinylated and Streptavidin- Peroxidase conjugated second specific anti-IGFBP-1-Antibody binds in turn to the immobilised IGFBP-1. Finally, the bound peroxidase catalyses the substrate reaction resulting in a colored product. Therefore colour intensity is highly specific and quantitatively depending on the IGFBP-1-level of the samples. Sample Types: serum, plasma, urine, saliva, cell culture. Range: 0.1 - 8ng/mL. Sensitivity: 0.02 ng/mL. Time: 1.75 hours • The Insulin-like Growth Factors I and - II are free in body fluids and tissues but are bound to specific binding proteins. Until today seven different binding proteins (IGFBP-1 to -7) can be differentiated additionally several IGFBP-related proteins have also been detected. Bioavailability of IGF is regulated by these IGFBPs or better their proteolytic cleavage which reduces affinity to IGF. But the IGFBPs as well as their proteolytic fragments can also exert IGF-independent effects, like influencing cell migration or proliferation. IGFBP-1 (Placental Protein 12) consists of 234 aminoacids and has a molecular weight of approximately 25kDa. The coding DNA region is located on chromosome 7 [1, 2]. IGFBP-1 is mainly synthesized by foetal and adult liver tissue and decidual endometrium. Intensity of Expression varies enduring menstruation with a maximal expression in the late secretory phase [3, 4]. Further IGFBP-1 expression seems to be regulated by Insulin concentration, with Insulin inhibiting the expression. Insulin regulation results in diurnal fluctuations of up to factor 10 [5]. IGFBP-1 is posttranslational modificated by phosphorylation of serine residues 101, 119 and 169. Phosphorylation has physiological relevance as it increases affinity of IGFBP-1 to IGF. In adult humans phosphorylated IGFBP-1 of the liver is the predominant form in circulation. IGFBP-1 produced by endometrial tissue is significantly less phosphorylated than the liver originated form [6]. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | E01 Insulin like growth factor IGFBP-1, IGF-II, IGF ASSAY EIA ELISA GROWTH IGF IMMUNOASSAY INSULIN INSULIN-LIKE KIT protein |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | E01 |
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| Order / More Info | IGFBP-1 ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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