| 产品详情 |
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| Antigenic Specificity | C-FOS |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | mouse, rat |
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| Isotype | n/a |
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| Format | serum |
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| Size | 100 µL |
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| Concentration | n/a |
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| Applications | Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The C-FOS Antibody was raised to synthetic peptide sequence corresponding to human c-fos (4-17) coupled to bovine thyroglobulin with glutaraldehyde. For induction of c-fos protein activity rats were injected with 1.0 ml of 1.5 M NaCl per 100 grams of body weight. Negative control rats were injected with the same volume of normal saline. The ImmunoStar c-fos antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat paraventricular nucleus and supraoptic nucleus using indirect immunofluorescent and biotin/avidin-HRP techniques. No labeling was seen in negative control rats. Recommended primary dilutions for these methods are 1/200-1/400 in PBS/0.3% Triton X-100 - FITC Technique and 1/4000-1/6000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Specificity of the antiserum was demonstrated by blockage of staining in experimental rats by omission of c-fos antibody or by substitution of antibody pre-incubated with synthetic peptide or the conjugate. Immunoblot analysis of mediobasal hypothalamus showed a single band of approximately 55-60 kD. |
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| Immunogen | Synthetic human c-fos (4-17) |
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| Other Names | n/a |
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| Gene, Accession # | n/a |
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| Catalog # | 26209 |
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| Price | |
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| Order / More Info | C-FOS Antibody from IMMUNOSTAR INC. |
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| Product Specific References | PubMed: 23262236, 23085313, 21933288, 20214438, 16533665, 15219664, 12676287, 12353219, 10362318 |
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