产品名称 Far-Western Blot Analysis from CREATIVE PROTEOMICS
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Product NameFar-Western Blot Analysis
DescriptionFar-WesternBlot is a molecular biological method, which is based on the technique of Westernblot, to detect in vitro protein-protein interactions, especially for detecting interactions that do not require the native structure of the protein of interest. The overall workflow of far-Western blot technique is similar to Western blotting, except that antibodies are needed for probing target proteins in Western blotting. Whereas in far-Western analysis, antibodies are not needed, instead a purified and labeled bait proteins are used to probe and detect a target prey protein on the membrane. In both Western blotting and far-Western blot, proteins are separated either SDS gel of native PAGE gel, followed by transferring proteins from the gel onto the membrane. After transfer, the membrane is blocked with BSA with empirical concentration.
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