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Antigenic Specificity | Lac I |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | E.coli and S.sonnei |
Isotype | n/a |
Format | immunoaffinity purified |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein. Specific conditions for reactivity and detection of Lac I should be optimized by the end user. Expect a band approximately ~38 kDa in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined. |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein. |
Other Names | rabbit anti-LacI antibody, Lac-I, Lac I, Lactose operon repressor, DNA binding transcriptional repressor antibody, Transcriptional repressor of the lac operon antibody |
Gene, Accession # | LacI, Gene ID: 8156885, NCBI: 146576, UniProt: P03023 |
Catalog # | 600-401-B04 |
Price | |
Order / More Info | Lac I Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Oh J, Chang KW, and Hughes SH (2006) Mutations in the U5 Sequences Adjacent to the Primer Binding Site Do Not Affect tRNA Cleavage by Rous Sarcoma Virus RNase H but Do Cause Aberrant Integrations In Vivo J.Virol 80(1):451-9 |