| 产品详情 |
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| Antigenic Specificity | AKT1, internal |
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| Clone | 14E5.A2.B2.H9 |
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| Host Species | Mouse |
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| Reactive Species | human |
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| Isotype | IgG2a kappa |
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| Format | Protein A purified |
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| Size | 1 Pair |
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| Concentration | 1.0 mg/ml |
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| Applications | ELISA, Flow Cytometry, Immunohistochemistry, Western Blot. Anti-AKT1 Antibody with the matched secondary are suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This primary monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This primary and secondary antibody pair has been carefully chosen to give best resutls. Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. This antibody pair is sufficient for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2). |
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| Immunogen | Anti-AKT1 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein. Anti-Mouse IgG HRP secondary antibody was produced by repeated immunizations with Mouse IgG whole molecule |
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| Other Names | AKT 1 antibody pair, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, primary and secondary antibody pair, Primary+Secondary pair, matched antibody pair |
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| Gene, Accession # | AKT1, Gene ID: 207, NCBI: NP_001014431.1, UniProt: P31749 |
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| Catalog # | K-I51 |
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| Price | |
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| Order / More Info | AKT1, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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