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Antigenic Specificity | AKT pS473, C-terminus |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, mouse, rat |
Isotype | n/a |
Format | immunoaffinity purified |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, Flow Cytometry, Immunohistochemistry, Western Blot. This antibody is phospho specific for pS473 and is suitable for western blotting, immunohistochemistry (formalin-fixed paraffin-embedded sections), immunofluorescence and ELISA. By immunoblot a single band of the expected apparent molecular weight is observed. For immunohistochemistry no pre-treatment of sample is required. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKTpS473. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested. |
Immunogen | This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a C-terminus region near aa 460-480 of human, mouse, rat and chicken AKT proteins conjugated to KLH. |
Other Names | rabbit anti-AKT pS473 Antibody, AKT1 phospho S473, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1 |
Gene, Accession # | AKT1, Gene ID: 207, NCBI: 62241011, UniProt: P31749 |
Catalog # | 600-401-268 |
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Order / More Info | AKT pS473, C-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Henle SJ, Wang G, Liang E, Wu M, Poo MM, Henley JR. (2011) Asymmetric PI(3,4,5)P3 and Akt signaling mediates chemotaxis of axonal growth cones. J Neurosci. 2011 May 11;31(19):7016-27. doi: 10.1523/JNEUROSCI.0216-11.2011. |
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