| 产品详情 |
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| Product Name | Glutathione Detection Kit |
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| Description | The Glutathione Fluorescent Detection kit is designed to quantitatively measure glutathione (GSH), and oxidized glutathione (GSSG) present in a variety of samples. The kit is unique in that both free and oxidized glutathione are detected in the same well in the microtiter plate. No separation or washing is required. Total glutathione is the sum of GSSG plus GSH. A GSH standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. The kit utilizes a proprietary non-fluorescent molecule, StressXpress® Detection Reagent, that will covalently bind to the free thiol group on GSH to yield a highly fluorescent product. After mixing the sample or standard with the Detection Reagent and incubating at room temperature for 15 minutes, the fluorescent product is read at 510 nm in a fluorescent plate reader with excitation at 390 nm. The concentration of the GSH in the sample is calculated, after making a suitable correction for any dilution of the sample, using software available with most fluorescence plate readers. Free glutathione, GSH, is read first after 15 minutes, followed by addition of a reaction mixture that converts all the oxidized glutathione, GSSG, into free GSH, which then reacts with the excess Detection Reagent to yield the signal related to Total GSH content. The total concentration of GSH generated in the sample is calculated from the generated signal. We have provided a 96 well plate for measurement but this assay is adaptable for higher density plate formats. The end user should ensure that their HTS black plate is suitable for use with these reagents prior to running samples. Direct Quantitative Assay. Intra Assay Precision:Two each of SSA treated human urine and whole blood samples were further diluted in 1% SSA Sample Diluent and run in replicates of 20 in an assay. The mean and precision of the calculated GSH concentrations were: Sample 1 (Free)- 1.27 µM, 4.0% CV, Sample 1 (Total)- 2.30 µM, 4.7% CV Sample 2 (Free)- 2.00 µM, 3.1% CV, Sample 2 (Total)- 3.80 µM, 4.7% CV Sample 3 (Free)- 8.33 µM, 4.6% CV, Sample 3 (Total)- 9.77 µM, 2.7% CV Sample 4 (Free)- 3.89 µM, 3.0% CV, Sample 4 (Total)- 4.45 µM, 2.3% CV Inter Assay Precision: Two each of SSA treated human urine and blood samples were further diluted in 1% SSA Sample Diluent and run in duplicates in twenty assays run over multiple days by two operators. The mean and precision of the calculated GSH concentrations were: Sample 1 (Free)- 1.30 µM, 8.6% CV, Sample 1 (Total)- 2.40 µM, 9.3% CV Sample 2 (Free)- 1.83 µM, 14.7% CV, Sample 2 (Total)- 3.57 µM, 10.0% CV Sample 3 (Free)- 9.38 µM, 6.0% CV, Sample 3 (Total)-11.67 µM, 6.0% CV Sample 4 (Free)- 4.89 µM, 7.2% CV, Sample 4 (Total)- 5.89 µM, 8.0% CV. Range: 0.195 - 25 uM. Sensitivity: 45 nM in the Free GSH and 48 nM in the Total GSH. |
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| Size | 96 well |
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| Concentration | n/a |
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| Applications | Fluorometric detection assay to measure the total GSH content in samples. RUO |
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| Other Names | StressXpress® γ-L-Glutamyl-L-cysteinylglycine Detection Kit(2S)-2-Amino-5-[[(2R)-1-(carboxymethylamino)-1-oxo- 3-sulfanylpropan-2-yl]amino]-5-oxopentanoic acid Detection Kit |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | SKT-202-96 |
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| Order / More Info | Glutathione Detection Kit from STRESSMARQ BIOSCIENCES INC. |
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| Product Specific References | n/a |
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