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| Product Name | Glutathione S-Transferase Activity Kit |
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| Description | The Glutathione S-Transferase Activity kit is designed to quantitatively measure the activity of GST present in a variety of samples. A GST standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. The kit utilizes a non-fluorescent molecule that is a substrate for the GST enzyme which covalently attaches to glutathione (GSH) to yield a highly fluorescent product. Mixing the sample or standard with the supplied Detection Reagent and GSH and incubating at room temperature for 30 minutes yields a fluorescent product which is read at 460 nm in a fluorescent plate reader with excitation at 390 nm. The activity of the GST in the sample is calculated, after making a suitable correction for any dilution of the sample, using software available with most fluorescence plate readers. We have provided a 96 well plate for measurement but this assay is adaptable for higher density plate formats. The end user should ensure that their HTS black plate is suitable for use with these reagents prior to running samples. Direct Enzyme Activity Assay. Species Reactivity: human, mollusk, fish, toadfish (batrachoididae), oyster. Intra Assay Precision:Four serum samples were diluted 1:2 with Assay Buffer and run in replicates of 16 in an assay. The mean and precision of the calculated GST activities were: Sample 1- 315.9 mU/mL, 4.6% CV Sample 2- 221.2 mU/mL, 5.6% CV Sample 3- 88.2 mU/mL, 4.2% CV Sample 4- 22.7 mU/mL, 6.6% CV Inter Assay Precision: Four serum samples were diluted 1:2 with Assay Buffer and run in duplicates in twenty assays over multiple days by four operators. The mean and precision of the calculated GST activities were: Sample 1- 291.7 mU/mL, 12.6% CV Sample 2- 218.5 mU/mL, 11.0% CV Sample 3- 89.6 mU/mL, 10.4% CV Sample 4- 23.0 mU/mL, 15.9% CV. Range: 7.81 - 500 mU/ml. Sensitivity: 2.70 mU/ml. Time: 30 minutes. |
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| Size | 96 well |
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| Concentration | n/a |
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| Applications | Fluorometric assay used to quantitatively measure the activity of glutathione S-transferase in samples. RUO |
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| Other Names | StressXpress® γ-L-Glutamyl-L-cysteinylglycine Activity Kit(2S)-2-Amino-5-[[(2R)-1-(carboxymethylamino)-1-oxo- 3-sulfanylpropan-2-yl]amino]-5-oxopentanoic acid Activity Kit |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | SKT-203-96 |
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| Order / More Info | Glutathione S-Transferase Activity Kit from STRESSMARQ BIOSCIENCES INC. |
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| Product Specific References | n/a |
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