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| Product Name | Retinol Binding Protein Urinary EIA Kit |
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| Description | The Retinol Binding Protein Urinary EIA Kit is designed to quantitatively measure RBP present in urine samples. A RBP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A RBP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of the RBP polyclonal antibody to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound RBP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the RBP in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers. Sandwich EIA (Enzyme Immunoassay). Species Reactivity: human, rat, dog, monkey. Intra Assay Precision: Four human urine samples were diluted 1:2 with Assay Buffer and run in replicates of 8 in an assay. The mean and precision of the calculated RBP concentrations were: Sample 1- 14.8 ng/mL, 4.5% CV Sample 2- 28.0 ng/mL, 7.5% CV Sample 3- 53.5 ng/mL, 7.3% CV Sample 4- 323.7 ng/mL, 2.1% CV Inter Assay Precision: Four human urine samples were diluted 1:2 with Assay Buffer and run in duplicates in twenty-one assays run over multiple days by three operators. The mean and precision of the calculated RBP concentrations were: Sample 1- 14.4 ng/mL, 9.1% CV Sample 2- 28.0 ng/mL, 9.2% CV Sample 3- 50.8 ng/mL, 8.1% CV Sample 4- 309.2 ng/mL, 14.0% CV. Range: 3.906 - 1000 ng/ml. Sensitivity: 2.90 ng/ml. Time: 90 minutes. |
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| Size | 96 well |
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| Concentration | n/a |
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| Applications | EIA kit used to measure RBP levels in urine samples. RUO |
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| Other Names | StressXpress® Cellular retinol binding protein EIA Kit, CRBP EIA Kit, CRBP EIA Kit, CRBP1 EIA Kit, CRBP2 EIA Kit, RBP1 EIA Kit, RBP2 EIA Kit, RBP4 EIA Kit, RBPC EIA Kit, Retinol binding protein 1 EIA Kit, Retinol binding protein 2 EIA Kit, Retinol binding protein 4 EIA Kit |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | SKT-218-96 |
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| Order / More Info | Retinol Binding Protein Urinary EIA Kit from STRESSMARQ BIOSCIENCES INC. |
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| Product Specific References | n/a |
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