| 产品详情 |
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| Product Name | Human HS apoA1 ELISA Kit |
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| Description | This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for apoA1 has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any apoA1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for apoA1 is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of apoA1 bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of apoA1 in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: brain extract, serum, plasma, cell culture supernatants. Range: 1.56-100 pg/ml. Sensitivity: 0.28 pg/mL. Time: 3 hours • Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Apolipoprotein A1, apoA1 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ARG80132 |
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| Order / More Info | Human HS apoA1 ELISA Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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