| 产品详情 |
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| Product Name | IGF-I ELISA |
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| Description | In order to dissociate IGF-I from the IGFBPs, the samples must be diluted in an acidic buffer (Sample Buffer PP). The diluted samples are then pipetted into the assay wells. The IGF-I antiserum is dissolved in a buffer, which is able to neutralize the acidic samples. After the IGF-I antibody solution has neutralized the samples, the present excess IGF-II occupies the IGF-binding sites of the binding proteins, thus allowing the measurement of the resulting free IGF-I. With this method, the IGFBPs are not removed, but their function and therefore their interference in the assay is neutralized. Due to the extremely low cross-reactivity of the IGF-I antibody with IGF-II, the excess of IGF-II does not disturb the interaction of the first antibody with IGF-I. Sample Types: serum, plasma, urine, saliva, cell culture. Range: 2 - 50 ng/ml. Sensitivity: 0.11 ng/mL. Time: 1.75 hours • Insulin-like growth factors (IGF) -I and -II play a pivotal role in regulating the proliferation, differentiation and specific functions of many cell types (1-3). IGF-I is identical with Somatomedin C (Sm-C) (4) and has a molecular weight of 7649 daltons (5). Its major regulators are growth hormone (GH) and nutrition (6), although its production in specific tissues is affected by a multitude of tropic hormones and other peptide growth factors. In contrast to many other peptide hormones, IGFs are avidly bound to specific binding proteins (IGFBP). The seven classes of IGFBPs which are known at present (7,8,22) either bind IGF-I and IGF-II with similar affinities or show a preference for IGF-II (9,10).A major problem of IGF-I measurement results from the interference of IGFBPs in the assay. Direct determinations in untreated serum samples (11) give false values because of the extremely slow dissociation of the IGF-I/IGFBP-3 complexes during the assay incubation. Depending on the ratio IGF-I to IGFBP the following errors may occur. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | 1 ASSAY EIA ELISA GROWTH IGF IGF-1 IGF1 IGF 1 IMMUNOASSAY INSULIN INSULIN-LIKE KIT mediagnost |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | E20 |
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| Order / More Info | IGF-I ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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