| 产品详情 |
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| Product Name | TESTOSTERONE ELISA |
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| Description | In the Testosterone ELISA Assay kit, the Ttestosterone in the blood is bound to SHBG (60%) and in lower quantity to other proteins (for example albumin); the unbound Testosterone (< 1% of total Testosterone) is known as ``free Testosterone''. The chemical formulation of this assay allows to release completely the Testosterone from bound proteins; thus Diametra Testosterone kit allows to measure the concentration of total Testosterone (bound + free) in the sample. For the measurement of free Testosterone only, ELISA Diametra ``Free Testosterone'' kit is available. Testosterone (Antigens) in the sample competes with the Antigensic Testosterone conjugated with horseradish peroxidase (HRP) present in the Conjugate for binding to the antibodies anti-testosterone coated on the microplates (solid phase). After the incubation, the bound/free separation is performed by a simple solid-phase washing. The enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blu color that changes into yellow when the Stop Solution (H2SO4) is added. The colour intensity is inversely proportional to the Testosterone concentration in the sample. Testosterone concentration in the sample is calculated through a calibration curve. Sample Types: serum, plasma. Range: 0.2 - 16 ng/ml. Sensitivity: 0.07 ng/mL. Time: 1.5 hours • Testosterone (17β-OH-4-androstene-3-one) is a steroid hormone family of androgens mainly produced by the Leydig cells located in the testes and, minimally, by the ovaries and the adrenal cortex. It is also present in women who, compared to men, have a greater tendency to convert into estrogen this hormone. In postpubertal males, testosterone is secreted primarily by the testes with only a small amount derived from peripheral conversion of androstenedione. In humans is deputy to the development of the sexual organs (differentiation of the testis and the whole genital apparatus) and of secondary sexual characteristics, such as beard, hair distribution, the timbre of the voice and muscles. The testosterone, during puberty, is also involved on skeletal development, limiting the elongation of the long bones and avoiding, in this way, a disproportionate growth of the limbs. In adult humans, the levels of testosterone have a very important role as regards the sexuality, the musculoskeletal system, the vitality and good health (mainly understood as protection from metabolic diseases such as hypertension and diabetes mellitus); helps to ensure fertility, as it stimulates the maturation of sperm in the testes. Also influence the quality and quantity of sperm produced, since the seminal work on the streets and on the prostate, deputies to the production of sperm. Daily production of testosterone in men varies from 5 to 7 milligrams, but exceeded 30 years, tends to decrease annually by 1%. In adult women over 50% of serum testosterone is derived from peripheral conversion of androstenedione secreted by the adrenal and ovary, with the remainder from direct secretion of testosterone by these glands. The majority of circulating testosterone is bound by SHBG and a smaller portion is bound by albumin. Only a small percentage (< 1%) exists in circulation as unbound or free testosterone. Testosterone effects can be classified as virilizing and anabolic effects, although the distinction is somewhat artificial, as many of the effects can be considered both. Anabolic effects include growth of muscle mass and strength, increased bone density and strength, and stimulation of linear growth and bone maturation. Virilizing effects include maturation of the sex organs, and after birth (usually at puberty) a deepening of the voice, growth of the beard and axillary hair (male secondary sex characteristics). Testosterone levels decline gradually with age in men (andropause). The signs and symptoms are non-specific, and are generally associated with aging such as loss of muscle mass and bone density, decreased physical endurance, decreased memory ability and loss of libido. In females of all ages, elevated testosterone levels can be associated with a variety of virilizing conditions, including adrenal tumors and polycystic ovarian disease. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Testosterone, steroid hormones |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | DCM002 |
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| Order / More Info | TESTOSTERONE ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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