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Product Name | Tg (Thyroglobulin) Ultrasensitive ELISA |
Description | Tg of serum samples, controls and calibrators react with the solid phase bound anti-hTg antibody during the first incubation period of 2.5 hours at room temperature. After removal of the non-bound components by a washing step anti-hTg HRP conjugate interacts with the Tg molecules trapped by the immobilized anti-hTg antibody during the second incubation at room temperature over night (> 15 hours). The sandwich type com plexes formed are separated from unbound material by a further washing step. HRP converts the colorless substrate solution of 3,3',5,5'-tetramethylbenzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 15 min, turning the solution from blue to yellow. Sample Types: serum. Range: 0.03 - 3 ng/mL. Sensitivity: 0.03 ng/mL. Time: overnight • Biochemically, Tg is to be understood as a rather complex family of molecules. It is micro heterogeneous with inter- and intra-individual variations (iodination degree, carbohydrate contents etc.). Dimers and several fragments also exist. Additional heterogeneity is due to malignant de-differentiation. Specifically and unspecifically interfering factors in individual sera cause further problems. Therefore, the Tg determination still represents a rather ambitious method. |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Tg, Thyroglobullin, Thyroid, |
Gene, Accession, CAS # | n/a |
Catalog # | TGR31-K01 |
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Order / More Info | Tg (Thyroglobulin) Ultrasensitive ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |
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