| 产品详情 |
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| Product Name | Total Glutathione Assay Kit |
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| Description | The Total Glutathione (tGSH) Microplate Assay kit employs a kinetic enzymatic recycling assay based on the oxidation of GSH by 5,5'-dithiobis(2-nitrobenzoic acid) [DTNB] to measure the total glutathione (tGSH) content of biological samples. The included glutathione standards or treated samples are added to the microtiter plate wells, followed by DTNB and glutathione reductase. Addition of b-NADPH2 to the wells initiates the progressive reduction of DTNB by GSH, causing a color increase that is monitored at 405 nm. The rate of color change, followed over a 5 minute time period, is proportional to the tGSH concentration. Consequently, the concentration of tGSH in unknown samples may be determined by reference to the standard curve. GSH reacts with DTNB to produce a colored ion, which absorbs light at 405 nm, and a mixed disulphide. This disulphide reacts with further quantities of GSH present to liberate another ion and GSSG. GSSG is reduced enzymatically to GSH which then re-enters the cycle (see figure below). Since GSSG represents only a small percentage of total acid-solution free glutathione, the resulting values for tGSH (which encompasses both GSH and GSSG) are expressed in units of GSH equivalents. Sample Types: biological fluids. Range: 0.5 - 20 µM. Sensitivity: 0.5 µM. Time: 20 minutes • Glutathione (g-glutamylcysteinylglycine or GSH) is a naturally occurring tripeptide whose nucleophilic and reducing properties play a central role in metabolic pathways, as well as in the antioxidant system of most aerobic cells. GSH is required as a coenzyme by a variety of enzymes including glutathione peroxidase, glutathione S-transferase and thioltransferase. GSH also plays a major role in drug metabolism, calcium metabolism, the g-glutamyl cycle, cell membrane and blood platelet functions. GSH is crucial to a variety of life processes, including the detoxification of xenobiotics, maintenance of the -SH level of proteins, thiol-disulfide exchange, removal of hydroperoxides and free radicals, and amino acid transport across membranes. Physiological values for the concentration of intracellular GSH generally range from 1 to 10 mM. Although many methods have been described for the assay of GSH, most of the reliable ones have been labor intensive. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Glutathione, GSH, TGSH, Total Glutathione, |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | GLU39-K01 |
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| Order / More Info | Total Glutathione Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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