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| Product Name | Universal Protease Activity ELISA |
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| Description | The Eagle Biosciences Universal Protease Activity ELISA Assay Kit measures activity of proteases like matrix metalloproteases (MMPs), A Disintegrin And Metalloproteinase with Thrombospondin Motifs (ADAMTS), especially High temperature requirement factor A (HtrA). It consists of two modules: Protease module and the ELISA module. In this assay, a biotinylated β-Casein coupled beads is first digested with protease(s)/HtrA1. The proteolytic cleavage of the substrate releases a biotinylated β-Casein fragment, without beads, that will bind on streptavidin-coated plate and can be further quantified with one specific monoclonal antiβ-Casein antibodies and with a Peroxidase-labeled antibody The Eagle Biosciences Universal Protease Activity ELISA Assay Kit is for research use only and not to be used in diagnostic procedures. Range: 0.312 - 20 nM. Sensitivity: 312 pM HtrA1. Time: 2.25 hours • Proteases play important roles in the control of multiple biological processes in all living organisms. They regulate the activities of many proteins, modulate protein-protein interactions, create new bioactive molecules, contribute to the processing of cellular information and generate, transduce and amplify molecular signals. As a direct result of these multiple actions, proteases influence DNA replication and transcription, cell proliferation and differentiation, tissue morphogenesis and remodelling, heat shock and unfolded protein responses, angiogenesis, neurogenesis, ovulation, fertilization, wound repair, stem cell mobilization, haemostasis, blood coagulation, inflammation, immunity, autophagy, senescence, necrosis and apoptosis. Consistent with these essential roles of proteases in cell behaviour, survival and death of all organisms, alterations in proteolytic systems underlie multiple pathological conditions such as cancer, neurodegenerative disorders, inflammatory and cardiovascular diseases. The Universal Protease Activity ELISA is based on a universal substrate for proteases, beta casein which is labeled with biotin and coupled on beads. The cleaved fragments of biotinylated beta casein are detected with an enzyme linked immuno assay (ELISA). The combinations of bead technology and ELISA have a synergistic effect. The sensitivity and specificity via antibodies is a feature of ELISA and the beads have no scale limit and can be used in variable volumes. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | established for quantitative activity determination for HtrA-, MMP-, ADAMTS-Protease among other, using HtrA1 as standard. RUO |
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| Other Names | 30 516 103, 30516103, ANTIBODY ASSAY EIA ELISA Universal Protease IMMUNOASSAY TEST, HtrA, MMP |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 30 516 103 |
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| Order / More Info | Universal Protease Activity ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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