产品详情 |
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Antigenic Specificity | Ki-67, C-terminus |
Clone | SP6 |
Host Species | Rabbit |
Reactive Species | human, rat |
Isotype | IgG |
Format | supernatant |
Size | 100 µL |
Concentration | n/a |
Applications | ELISA, Immunohistochemistry, Western Blot. This monoclonal antibody contains TBS/1% BSA and is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 345 and 395 kDa in size corresponding to Ki-67 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min. Ki-67 is a nuclear protein. Tonsil tissue can be used as a positive control. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This product is clarified tissue culture supernatant. The antibody is specific for human Ki-67 protein. Cross-reactivity occurs with rat Ki-67. Cross reactivity with Ki-67 protein from other sources has not been determined. |
Immunogen | This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminus of human Ki-67 protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology. |
Other Names | rabbit anti-Ki-67 Antibody, MKI67, Ki-67 nuclear protein |
Gene, Accession # | MKI67, Gene ID: 4288, NCBI: CAI16903.1, UniProt: P46013 |
Catalog # | 900-C01-B38 |
Price | |
Order / More Info | Ki-67, C-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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