| 产品详情 |
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| Antigenic Specificity | Fibronectin |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human, mouse, rat, bovine, monkey |
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| Isotype | n/a |
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| Format | biotin conjugate |
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| Size | 100 µg |
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| Concentration | 1.0 mg/ml |
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| Applications | ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-fibronectin (rabbit) antibody was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 µg of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Anti-fibronectin (rabbit) antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificity. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible. |
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| Immunogen | Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE. |
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| Other Names | rabbit anti-Fibronectin antibody biotin conjugation, biotin conjugated rabbit anti-Fibronectin antibody, FN1, FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3 |
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| Gene, Accession # | FN1, Gene ID: 2335, NCBI: AAA53376.1, UniProt: P02751 |
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| Catalog # | 600-406-117 |
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| Price | |
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| Order / More Info | Fibronectin Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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