产品详情 |
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Antigenic Specificity | LDB1 |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | mouse, human, rat |
Isotype | n/a |
Format | immunoaffinity purified |
Size | 100 µg |
Concentration | 1.80 mg/mL |
Applications | ELISA, Chip, Western Blot. This affinity purified antibody has been tested for use in ELISA, western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene; a putative LDB1 binding region as suggested by Orkin et al. Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells. We suggest using 20µg for 10E8 cells for ChIP. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This affinity purified antibody is directed against the mouse LDB1protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, frog, chicken and rat based on 100% homology for the immunogen sequence. Cross reactivity with LDB1 homologues from other sources has not been determined. |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 361-373 of mouse LDB1 protein. |
Other Names | rabbit anti-LDB1 antibody, rabbit anti-LDB1/CLIM2 antibody, rabbit anti-CLIM2 antibody, LDB-1, CLIM-2, LIM domain-binding protein 1, Carboxyl-terminal LIM domain-binding protein 2, LIM domain-binding factor CLIM2, Nuclear LIM interactor |
Gene, Accession # | LDB1, Gene ID: 16825, NCBI: 6754520, UniProt: P70662 |
Catalog # | 600-401-350 |
Price | |
Order / More Info | LDB1 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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