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| Product Name | Fab-pHast human |
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| Description | Fab-pHast human is one of our fastest tools for quantitative testing of your primary antibody's specificity, binding, and internalization, providing results in 1 day. Fab-pHast human binds to your primary human antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. A successful assay will provide an EC 50 by way of a fluorescence detecting plate reader, illuminating your lead antibody candidates. Specificity and Preparation: This secondary conjugate recognizes YOUR human monoclonal antibody. Fab-pHast is a chemical conjugate of goat anti-human monovalent antibody and a pH-dependent fluorescent reporter. The antibody fragments used to make Fab-pHast human are affinity-purified polyclonal antibodies against both the heavy and light chain of human IgG. The antibody fragment used in this product will cross-react across immunoglobulin classes and subclasses of the same species as they share the same light chain (either kappa or lambda). The pHast fluorescent dye has an excitation wavelength of 532 nm with an emission maxima at 560 nm. |
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| Size | 100 ug |
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| Concentration | n/a |
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| Applications | Fab-pHast human generates quantitative testing of the specific, binding, and internalization of your primary human antibody, with results in 1-day. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize. |
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| Other Names | pH-dependent fluorescent reporter |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | PH-01-100 |
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| Order / More Info | Fab-pHast human from ADVANCED TARGETING SYSTEMS |
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| Product Specific References | n/a |
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