| 产品详情 |
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| Antigenic Specificity | ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) |
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| Clone | 1503310 |
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| Host Species | Mouse |
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| Reactive Species | human |
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| Isotype | IgG1, κ |
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| Format | PerCP-Cyanine5.5 conjugate |
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| Size | 100 tests |
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| Concentration | n/a |
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| Applications | Intracellular Staining for Flow Cytometry (ICFC) |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | PerCP/Cyanine5.5 anti-ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352). ZAP70 was identified in TCR-stimulated Jurkat cells. It is an inactive cytosolic tyrosine kinase that is recruited to a transmembrane receptor lacking intrinsic catalytic activity. ZAP70 and the related spleen tyrosine kinase (Syk) play a critical role in T-cell development and activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. ZAP70 activation can be regulated by binding to phosphorylated ITAMs of the TCR and by phosphorylation of multiple tyrosine residues on ZAP70. Phosphorylation of Tyr315 and Tyr319 are essential for ZAP70 positive regulation of T-lymphocyte activation whereas Tyr292 has a negative regulatory role.Phosphorylated Tyr319 is a positive regulator of ZAP70, which triggers the binding of Lck and activation of NFAT and IL-2 induction. The binding of Lck to phosphorylated Tyr319 promotes Lck mediated phosphorylation of Tyr493, and facilitates activation of downstream signaling. The importance of Tyr319 phosphorylation in positive regulation of ZAP70 was confirmed in a mouse model with Tyr319F ZAP70 mutant expression, which resulted in severe defects in calcium mobilization and thymocyte selection. |
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| Immunogen | Modified peptide. |
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| Other Names | Phospho Zeta-Chain (TCR) Associated Protein Kinase 70 kD, Phospho SRK, Phospho 70 KD Zeta-Chain Associated Protein, Phospho Tyrosine-protein kinase ZAP-70 |
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| Gene, Accession # | Gene ID: 7535 |
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| Catalog # | 683710 |
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| Price | |
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| Order / More Info | ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody from BIOLEGEND |
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| Product Specific References | n/a |
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