| 产品详情 |
| Edit |   |
|---|
| Antigenic Specificity | GFP |
|---|
| Clone | 9F9.F9 |
|---|
| Host Species | Mouse |
|---|
| Reactive Species | n/a |
|---|
| Isotype | IgG1 kappa |
|---|
| Format | Protein A purified |
|---|
| Size | 1 mg |
|---|
| Concentration | 1.0 mg/mL |
|---|
| Applications | ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot. Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. |
|---|
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
|---|
| Description | GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215) from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP. |
|---|
| Immunogen | Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. |
|---|
| Other Names | mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish |
|---|
| Gene, Accession # | n/a |
|---|
| Catalog # | 600-301-215 |
|---|
| Price | |
|---|
| Order / More Info | GFP Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
|---|
| Product Specific References | Yang F, Camp DG 2nd, Gritsenko MA, et al. Identification of a novel mitotic phosphorylation motif associated with protein localization to the mitotic apparatus. J. Cell. Sci. 2007 Nov 15;120(Pt 22):4060-4070. Shen W, Da Silva JS, He H, Cline HT. Type A GABA-receptor-dependent synaptic transmission sculpts dendritic arbor structure in Xenopus tadpoles in vivo. J. Neurosci. 2009 Apr 15;29(15):5032-5043.Koenig A, Mueller C, Hasel C, et al. Collagen type I induces disruption of E-cadherin-mediated cell-cell contacts and promotes proliferation of pancreatic carcinoma cells. Cancer Res. 2006 May 1;66(9):4662-4671. Jesse S, Koenig A, Ellenrieder V, Menke A. Lef-1 isoforms regulate different target genes and reduce cellular adhesion. Int. J. Cancer. 2010 Mar 1;126(5):1109-1120. Goel M, Sienkiewicz AE, Picciani R, et al. Cochlin induced TREK-1 co-expression and annexin A2 secretion: role in trabecular meshwork cell elongation and motility. PLoS ONE. 2011;6(8):e23070. Forrest JC, Paden CR, Allen RD, et al. ORF73-Null Murine Gammaherpesvirus 68 Reveals Roles for mLANA and p53 in Virus Replication. J Virol. 2007 Nov;81(21):11957-11971.Manik Goel, Adam E. Sienkiewicz, Renata Picciani, Jianhua Wang, Richard K. Lee, Sanjoy K. Bhattacharya. (2012) Cochlin, Intraocular Pressure Regulation and Mechanosensing. PLOS/doi.org/10.1371/journal.pone.0034309.Terrell JL, Wu HC, Tsao CY, Barber NB, Servinsky MD, Payne GF, Bentley WE. (2015) Nano-guided cell networks as conveyors of molecular communication. Nat Commun. 2015 Oct 12;6:8500. doi: 10.1038/ncomms9500.Feijs KL, Kleine H, Braczynski A, Forst AH, Herzog N, Verheugd P, Linzen U, Kremmer E, Luescher B. (2013) ARTD10 substrate identification on protein microarrays: regulation of GSK3? by mono-ADP-ribosylation. Cell Commun Signal. 2013 Jan 19;11(1):5. doi: 10.1186/1478-811X-11-5. |
|---|
| 产品资料 |
|
|