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Antigenic Specificity | RFP |
Clone | polyclonal |
Host Species | Goat |
Reactive Species | n/a |
Isotype | n/a |
Format | IgG fraction |
Size | 25 µL |
Concentration | 1 mg/mL |
Applications | ELISA, IF Microscopy, Western Blot. Polyclonal anti-RFP is designed to detect RFP and its variants. Optimal titers for applications should be determined by the researcher. Expect a band ~27kDa in Western Blot in the appropriate cell lysate or extract. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-RFP (GOAT) is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. |
Immunogen | The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma. |
Other Names | goat anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583 |
Gene, Accession # | DsRed |
Catalog # | 200-101-379S |
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Order / More Info | RFP Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Takeda N, Jain R, Leboeuf MR, Padmanabhan A, Wang Q, Li L, Lu MM, Millar SE, Epstein JA.. (2013) Hopx expression defines a subset of multipotent hair follicle stem cells and a progenitor population primed to give rise to K6+ niche cells. Development. 2013 Apr;140(8):1655-64. |
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