| 产品详情 |
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| Antigenic Specificity | RFP |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | n/a |
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| Isotype | n/a |
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| Format | affinity purified |
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| Size | 25 µL |
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| Concentration | 0.95 mg/mL |
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| Applications | ELISA, Immunohistochemistry, IF Microscopy, Western Blot. Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This product was prepared from monospecific antiserum by imunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal. |
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| Immunogen | The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma. |
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| Other Names | rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583 |
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| Gene, Accession # | DsRed |
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| Catalog # | 600-401-379S |
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| Price | |
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| Order / More Info | RFP Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Takeda N, Jain R, Leboeuf MR, Padmanabhan A, Wang Q, Li L, Lu MM, Millar SE, Epstein JA.. (2013) Hopx expression defines a subset of multipotent hair follicle stem cells and a progenitor population primed to give rise to K6+ niche cells. Development. 2013 Apr;140(8):1655-64.Ortega F, Gascon S, Masserdotti G, Deshpande A, Simon C, Fischer J, Dimou L, Chichung Lie D, Schroeder T, Berninger B. (2013) Oligodendrogliogenic and neurogenic adult subependymal zone neural stem cells constitute distinct lineages and exhibit differential responsiveness to Wnt signalling. Nat Cell Biol. 2013 Jun;15(6):602-13. doi: 10.1038/ncb2736. Epub 2013 May 5.Tian X, Hu T, He L, Zhang H, Huang X, Poelmann RE, Liu W, Yang Z, Yan Y, Pu WT, Zhou B. (2013) Peritruncal coronary endothelial cells contribute to proximal coronary artery stems and their aortic orifices in the mouse heart. PLoS One. 2013 Nov 21;8(11):e80857. doi: 10.1371/journal.pone.0080857. eCollection 2013. |
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