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| Antigenic Specificity | RFP |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | n/a |
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| Isotype | n/a |
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| Format | ready to use |
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| Size | 100 µL |
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| Concentration | sufficient to run 10 immunoblot tests |
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| Applications | ELISA, Immunohistochemistry, IF Microscopy, Immunoprecipitation, Western Blot. Ready-To-Use Anti-RFP is designed to detect RFP and its variants. Ready-To-Use Anti-RFP Rabbit Polyclonal Antibody has been optimized and validated in western blot using 1:1000 dilution. This Anti-RFP (RTU) Antibody is sufficient to run 10 western blots. Although not tested, this antibody is likely functional in ELISA, immunohistochemistry, immunofluorescence, and immunoprecipitation. Optimal titers for these applications should be determined by the researcher. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | RTU Anti-RFP was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. Fluorescent proteins such as green fluorescent protein (GFP) or Discosoma Red Fluorescent Protein (or DsRFP) are widely used in research practice. DsRed is isolated from sea anemone Discosoma sp. mushroom. GFP and DsRed share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, DsRed protein is similar to GFP protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, GFP protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Using site-directed mutagenesis, several DsRed protein variants have been created allowing the red fluorescent protein mature as a monomeric form. Among DsRFP monomeric variants are monomeric mutant mRFP1, mBanana, mCherry, mHoneydew, mPlum, mOrange, mStrawberry and mTangerine that offer a wide range of fluorescent colors. As Rockland RFP polyclonal antibodies are raised against whole RFP protein of wild type, the polyclonal antibodies are expected to recognize all RFP variant forms. This RFP antibody has been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein. All Rockland Immunochemical's RFP antibodies are affinity-purified to assure both high affinity and specificity. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays. |
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| Immunogen | The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma. |
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| Other Names | DsRed protein, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583, sea anemone Discosoma sp. Mushroom, RFP antibody, RFP-RTU, Ready To Use Antibody, RTU Antibody |
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| Gene, Accession # | DsRed |
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| Catalog # | 600-401-379-RTU |
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| Price | |
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| Order / More Info | RFP Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Takeda N, Jain R, Leboeuf MR, Padmanabhan A, Wang Q, Li L, Lu MM, Millar SE, Epstein JA.. (2013) Hopx expression defines a subset of multipotent hair follicle stem cells and a progenitor population primed to give rise to K6+ niche cells. Development. 2013 Apr;140(8):1655-64. Ortega F, Gascon S, Masserdotti G, Deshpande A, Simon C, Fischer J, Dimou L, Chichung Lie D, Schroeder T, Berninger B. (2013) Oligodendrogliogenic and neurogenic adult subependymal zone neural stem cells constitute distinct lineages and exhibit differential responsiveness to Wnt signalling. Nat Cell Biol. 2013 Jun;15(6):602-13. doi: 10.1038/ncb2736. Epub 2013 May 5. Tian X, Hu T, He L, Zhang H, Huang X, Poelmann RE, Liu W, Yang Z, Yan Y, Pu WT, Zhou B. (2013) Peritruncal coronary endothelial cells contribute to proximal coronary artery stems and their aortic orifices in the mouse heart. PLoS One. 2013 Nov 21;8(11):e80857. doi: 10.1371/journal.pone.0080857. eCollection 2013. |
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