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| Product Name | Phosphorylated Neurofilament NF-H ELISA Kit |
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| Description | This assay employs the sandwich enzyme immunoassay technique for the detection of pNF-H protein in plasma, serum, CSF and tissue extracts samples. pNF-H protein in samples and standards will bind to the capture mouse monoclonal antibody coated on the microtiter plate. After appropriate washing steps, HRP-conjugated anti-pNF-H monoclonal antibody binds to the captured protein. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of pNF-H protein bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of pNF-H protein in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: plasma, serum, csf and tissue extracts. Range: 0.156 - 10 ng/ml. Sensitivity: 70 pg/mL. Time: 3 hours • Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008] The pNF-H protein has been detected in large amounts following experimental spinal cord and brain injury in rats (18). Levels of greater than 100ng/mL of pNF-H were detectable in blood samples following serious spinal cord injury and lower but still easily detectable levels were seen in blood of animals given experimental brain injury. More recent studies have revealed considerable amounts of this protein in the blood of transgenic mice carrying mutations of human copper/zinc superoxide dismutase-1 which are associated with amyotrophic lateral sclerosis. These mice develop an axonal degeneration pathology similar to that seen in humans with ALS, and blood pNF-H levels can be used to monitor progression of the disease. Interestingly, pNF-H was detectable before the onset of obvious disease symptoms. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Neurofilament NF-H |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ARG81164 |
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| Order / More Info | Phosphorylated Neurofilament NF-H ELISA Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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