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| Product Name | 15-Epi-Lipoxin A4 ELISA Kit |
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| Description | The 15-Epi-Lipoxin A4 ELISA Kit (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of 15-epi-Lipoxin A4 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the 15-epi-LXA4 in the sample for a limited number of antibody binding sites. The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate that generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650 nm. The extent of color development is inversely proportional to the amount of 15-epi-LXA4 in the sample or standard. For example, the absence of 15-epi-LXA4 in the sample will result in a bright blue color, whereas the presence of 15-epi-LXA4 will result in decreased or no color development. Sample Types: biological fluids. Range: 0.02 - 2.0 ng/mL. Sensitivity: 0.02 ng/mL. Time: 2 hours • 15-epi-Lipoxin A4 (15-epi-LXA4) is an aspirin-triggered eicosanoid believed to be involved in the positive attributes of aspirin therapy for heart, cancer, and human immunodeficiency virus patients. During inflammation, neutrophils are activated. 15-epi-LXA4, when administered in vivo, inhibits neutrophil activation and dampens inflammation (Takano, 1997). 15-epi-LXA4 is naturally formed in the body. The development of this assay, which is both sensitive and selective for 15-epi-LXA4, will be essential to researchers studying anti-inflammatory medications. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | 15-epi-LXA4, 15-Epi-Lipoxin A4 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 15E39-K01 |
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| Order / More Info | 15-Epi-Lipoxin A4 ELISA Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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