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| Product Name | GLP-1 Total ELISA Assay Kit |
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| Description | Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 specific antibody and a horseradish peroxidase (HRP)-conjugated GLP-1 specific antibody is added to each well. After the first incubation period, a ``sandwich'' immunocomplex of ``Streptavidin - Biotin-Antibody - GLP-1(7-36)/(9-36) - HRP-conjugated antibody'' is formed and attached to the wall of the plate. The unbound HRP-conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36)/(9-36) on the wall of the microtiter well is directly proportional to the amount of Total GLP-1 in the sample. Sample Types: plasma. Range: 2 -54 pmol/L. Sensitivity: 0.6 pmol/L. Time: overnight • The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc. The Eagle Biosciences GLP-1 Total ELISA Assay Kit (enzyme-linked immunoassay kit) is intended quantitative determination of sum value of glucagon-like peptide-1 (7-36) and (9-36) [GLP-1 (7-36)] and (9-36) [GLP-1 (9-36)] in plasma samples. Eagle Biosciences GLP-1 Total ELISA Assay Kit is for research use only and not to be used in diagnostic procedures. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | GLP-1, glucagon-like-peptide-1 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | GP131-K01 |
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| Order / More Info | GLP-1 Total ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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