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| Product Name | Human Apolipoprotein AI and Apolipoprotein B Duplex ELISA Kit |
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| Description | This Human ApoAI and ApoB Duplex ELISA Kit employs the quantitative sandwich enzyme immunoassay technique for the detection and quantitation of both human ApoAI and ApoB (ApoB-100/48) in the same sample of plasma, serum or other biological fluids. Antibodies specific for ApoAI and ApoB has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any ApoAI and ApoB present are bound by the immobilized antibodies. After washing away any unbound substances, a biotin-conjugated antibody specific for ApoAI and an anti-ApoB antibody are added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Alkaline Phosphatase (AP) and a Horseradish Peroxidase conjugated secondary antibody reacts to anti-ApoB antibody are added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, an AP substrate solution is added to the wells and color develops in proportion to the amount of ApoAI bound in the initial step. The intensity of the color developed from AP substrate is measured at a wavelength of 405nm. After wash away the AP substrate, a HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of ApoB bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm. The concentration of ApoAI and ApoB in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: plasma, serum or other biological fluids. Range: 0.156 - 10 ng/ml (ApoAI); 1.56 - 100 ng/ml (ApoB). Sensitivity: 0.1 ng/ml (ApoAI); 1 ng/ml (ApoB). Time: 5 hours • Apolipoprotein AI (ApoAI) which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. [provided by RefSeq, Jul 2008] Apolipoprotein B (ApoB) occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. Apolipoprotein B is a major protein constituent of chylomicrons (apo B48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. [provided by RefSeq, Jul 2008 and uniprot] ApoA1 is often used as a biomarker for prediction of cardiovascular diseases. The ratio apoB-100/apoA1 (i.e. LDL & larger particles vs. HDL particles), NMR measured Lipoprotein (LDL/HDL) particle ratios has always had a stronger correlation with myocardial infarction event rates than older methods of measuring lipid transport in the water outside cells. [provided by wikipedia] A recent study indicated that determining the ratio of ApoB to ApoAI is the best predictor of those at risk of heart attack. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Apolipoprotein A1, APO A1, Apolipoprotein B APO B |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ARG81205 |
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| Order / More Info | Human Apolipoprotein AI and Apolipoprotein B Duplex ELISA Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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