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| Product Name | Human Apolipoprotein CIII ELISA Kit |
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| Description | This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for ApoCIII has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any ApoCIII present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for ApoCIII is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibodyenzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of ApoCIII bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of ApoCIII in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: plasma, serum or other biological fluids. Range: 31.3 - 2000 pg/ml. Sensitivity: 50 pg/ml. Time: 4.5 hours • Apolipoprotein CIII (ApoCIII) is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. [provided by RefSeq, Jul 2008] Apolipoprotein CIII (ApoCIII) is a component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Apolipoprotein CIII, APO CIII |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ARG81210 |
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| Order / More Info | Human Apolipoprotein CIII ELISA Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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