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| Product Name | Human BDNF ELISA Kit |
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| Description | The Eagle Biosciences BDNF (Brain-Derived Neurotrophic Factor) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for BDNF has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any BDNF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for BDNF is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm.The concentration of BDNF in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: serum, plasma, cell culture supernatants. Range: 15.625-1000 pg/ml. Sensitivity: 7 pg/mL. Time: 3.5 hours • The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. During development, BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), longterm depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | BDNF, Brain-Derived Neurotrophic Factor |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ARG81251 |
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| Order / More Info | Human BDNF ELISA Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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